whenever their normal growth was required. All isolates are
available at the host institution and processed for submission
in Microbial type culture collection-MTCC, Chandigarh, India.
To perform antimicrobial assay, the clinical isolates of
Escherichia coli ESBL and methicillin-resistant Staphylococcus
Aureus (MRSA) were obtained from the department of Medical
Microbiology ,Lund University, Lund (Sweden). Theywere
propagated in brain heart infusion (BHI,Oxoid) brothand
maintained as frozen stocksat 20 1C in BHI broth contain-
ing 10%(v/v) glycerol. Before use, frozen cultures were grown
on blood agar plate followed by two successive transfers into
BHI broth.