In this process, 100 g dry raw material is subjected to two extraction steps that yield over 90% of lutein (Vechpanich and Shotipruk, 2011). In each extraction step, liquid extraction used 500 mL hexane for 4 h at 40 C. After extraction, the mixture was left to stand for 20 min at room temperature to allow residues to settle. The hexane organic layer containing lutein is concentrated by a rotary evaporator at 40 C and the final extract is dried in vacuum drier at 30 C for 8 h. The remaining solid (marigold oleoresin) is collected and stored at 20 C. Starting with 100 g dry marigold flowers, approximate 10 g marigold oleoresin can be acquired