Watermelon is one of the tropical fruits which is often favored among all of the different types of fresh agricultural produce worldwide. For the commercial market, fresh and high-quality watermelons are harvested at a suitable maturity level based on the appearance of the fruit. With respect to this condition, the internal quality is required for monitoring the quality changes especially during storage. Physicochemical and environmental aspects are the main factors that could affect the quality of the fruit with regard to texture, sweetness, flavor, color, and shelf-life (Wani et al., 2014). Apart from that, the evaluation of fruit quality is crucial to determine the consumer’s choice when purchasing fresh watermelon, taking into account the quality grade and fruit maturity.