In all cases, increases in chlorophyll, carotenoids, and phenolics
were concurrent with the initiation of DET1 downregulation
and mimicked expression profiles until DET1 downregulation
was alleviated (Figures 1C to 1E). At this point, increased fruit
carotenoid, chlorophyll, and phenolic contents were maintained
even in the absence of DET1 downregulation. On the basis of the
expression and the concurrent appearance of the phenotype,
detailed characterization was initiated at the mature green
stages of fruit development (stage 5).