Fig. 5 shows the result of a successful voltage ramp experiment performed in the same way as described above (hyperpolarizing prepulses followed by positive-going ramps). Note that the reversal potential was again in the negative voltage range, indicating that Ca2 + passed the membrane more readily than chloride. This is in line with the results presented above. On average, the reversal potential was about − 6 mV independent of the polarity of the ramp (see also Table 2; media P7/B4).