Grape seeds were collected from grapes (Vitis vinifera L.) cv.
Tempranillo before and after making of wine. Seeds were ground in
a knife mill and powdered samples were sieved to select particles
between 0.5 to 3.0 mm, and they were stored at room temperature
until used in vacuum conditions. Dried grape seeds (GSEJ and
GSEW) were powdered and bioactive compounds were extracted
with water (80e90 C) for 5 h in Soxhlet. These extracts were
filtered and kept in a dark place at e80 C until the test.