A total of 50 cells were randomly chose and scored at IC25 concentration of Mn. After the scoring was done, the
percentages of cells with their respective scores were calculated (Fig. 4). 44% of the DNA damage were at score 0
and 6% of DNA damage were at score 4. Alkaline comet assay are one of the method developed to detect DNA
damage in cell. DNA strand breakages (double, single, alkali-labile sites expressed as single strand breaks) are able
to be detected by this comet assay which is also known as single-cell gel electrophoresis test [17]. According to
Collins [10], contamination of the environment with genotoxins can be assesed via alkaline comet assay with the
suitable organism that can be used as biosensors. In this study, genotoxicity of Mn on Acanthamoeba sp. was being
evaluated via comet assay. To assess the genotoxicity of this compound, the value of IC25 which is 12 ppm (half of
IC50 value) was used to treat the Acanthamoeba sp. cells for 2 hours at 30oC. IC25 value was used instead of IC50 to
determine whether Mn compound is able to induce genotoxic even at low concentration. The damage of the DNA
was evaluated according to five types of score which were 0, 1, 2, 3 and 4 (Fig. 4). They are categorized based on
A total of 50 cells were randomly chose and scored at IC25 concentration of Mn. After the scoring was done, thepercentages of cells with their respective scores were calculated (Fig. 4). 44% of the DNA damage were at score 0and 6% of DNA damage were at score 4. Alkaline comet assay are one of the method developed to detect DNAdamage in cell. DNA strand breakages (double, single, alkali-labile sites expressed as single strand breaks) are ableto be detected by this comet assay which is also known as single-cell gel electrophoresis test [17]. According toCollins [10], contamination of the environment with genotoxins can be assesed via alkaline comet assay with thesuitable organism that can be used as biosensors. In this study, genotoxicity of Mn on Acanthamoeba sp. was beingevaluated via comet assay. To assess the genotoxicity of this compound, the value of IC25 which is 12 ppm (half ofIC50 value) was used to treat the Acanthamoeba sp. cells for 2 hours at 30oC. IC25 value was used instead of IC50 todetermine whether Mn compound is able to induce genotoxic even at low concentration. The damage of the DNAwas evaluated according to five types of score which were 0, 1, 2, 3 and 4 (Fig. 4). They are categorized based on
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