Serum number 711 was used to probe antigen separated in the pH range 6–11 (Fig. 2b) and two very weakly reactive areas were found to correspond to protein spots on equivalent 2D-PAGE, stained for proteins.
These were identified as glutamate dehydrogenase (FTT_0380c) and sugar transamine/perosamine synthetase (FTT_1455c), as indicated in the protein stained gel image shown in Fig. 2e.
No single protein was observed to be immunoreactive with all Type A tularemia patient sera screened, although the proteins pyruvate dehydrogenase E2 (FTT_1484c) and ribosomal protein L7/L12 (FTT_0143) were observed to be reactive with ten of the twelve sera studied.
In addition, the proteins dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase (FTT_0077) and chaperonin protein DnaK (FTT_1269c) were reactive with 8 of the total 12 sera.