The freeze-dried snack bar samples (5 g) were mixed with CeliteTM
at a ratio of 1:1 w/w and then loaded into Dionex standard 33-
ml stainless steel extraction cells. A cellulose filter paper (30 mm,
Whatman) was placed at the end of the thimble. Extraction was
carried out under N2 in a pressurised multiple-sample extractor
(ASE 300, Dionex) (operating conditions: 60 C and 1500 psi with
5 min heating and 10 min static time). After four extraction cycles
were performed using n-hexane, the extracts were transferred to
pre-weighed Labconco glasses (LabConco Corporation) and dried
in the RapidVap concentrator unit at 60 C, 60% speed for
50 min. The Labconco glasses containing the extracted lipids were
weighed. The total lipid content was calculated as a percentage of
the snack bar sample.