High frequency direct shoot proliferation was induced in the shoot segments of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw.
on Murashige and Skoog's medium (MS) containing N6-benzyladenine (BA) or thidiazuron (TDZ) with the latter being more effective at 2.2–4.5 μM.
Shoots which developed on a TDZ containing medium elongated following transfer to a medium containing 2.2 μM BA and 10.8μM naphthaleneacetic acid (NAA).
Concentrations of TDZ above the optimal level had an inhibitory effect on shoot regeneration.
In both Dendrobium species the number of shoot bud formation was greatly influenced by expiant orientation.
Regenerated shoots were rooted on MS containing 10.8 μM indole-3-butyric acid (IBA). The plantlets were acclimated and eventually transferred to garden.