At the end of the feeding trial, th

At the end of the feeding trial, three fish from each aquarium were randomly collected, euthanized with an overdose of tricaine methanesulfonate (MS-222), pooled and stored at −80 °C for subsequent proximate analysis.

After descaling, fish from each aquarium were finely ground in a Hobart meat grinder and analyzed in duplicate for proximate composition following the standard methods (AOAC, 1990).

Moisture content was determined by drying samples in an oven at 100 °C until constant weight was reached.

Samples used for dry matter were digested with nitric acid and incinerated in a muffle furnace at 600 °C overnight for measurement of ash contents.

Protein was measured by combustion method using a FP-2000 Nitrogen Analyzer.

Lipid content of samples was determined by petroleum ether extraction using a Soxtec System.