Fiber determination
Duplicate samples of dried fruits, sugar extraction if containing >10% sugar were gelatinized with Trammel (heat stable α-amylase), and then enzymatically digested with protease and amyloglucosidase to remove protein and starch. Four volumes of ethanol were added to precipitate the solubilized dietary fibers. The solutions were allowed to stay at room temperate overnight. Total residue was filtered by washing with 78% ethanol 95% and acetone. After drying, the residue was weighed. One duplicate was analyzed for protein, and the other was incinerated at 525oC and was determined by weighting. Total dietary fiber was calculated as weighed residue-weighed protein ash(13).