Liquid Chromatography
A Shimadzu Prominence LC system containing a CBM-20A system controller, two LC-20AD pumps, a semi-micro gradient mixer SUS-20A, and a SIL-20AC autosampler was used. Separation
was performed on a GL Science Inertsil HILIC 5μm (150x3mm) column at a temperature of 40°C with a mobile phase of (A) acetonitrile + 10mM ammonium acetate and (B) water +
10mM ammonium acetate at a flow of 0.5mL/min. The gradient using normal phase conditions
An injection volume of 5μL was used.