Supernatant (100 µl) was diluted with 850 µl of 0.1 M sodium acetate buffer
(pH 4.5) incubated with 50 µl amyloglucosidase
(33 U/ml) at 50oC for 30 min in water bath After centrifuging at 2000xg for 10 min, the supernatant was retained for glucose determination.