That is, the level of protein in a cell is not always correlated with the expression of the gene. Therefore, the dynamic PPI network in this study can not perfectly match the real one. This finding may partially account for the inaccurate prediction of essential proteins. The contents of dynamic data are expected to increase in the future. The performance of our method can be improved by integrating several types of dynamic data to construct a realistic dynamic PPI network. Further efforts can also be exerted to improve prediction precision from the aspect of the inherent property of essential proteins.