It has seven nucleotides different from
Fig. 1. Schematic diagram representation of recombinant plasmids.
Dashed line represents genomic DNA of B. sphaericus 2297. Positions
of A–F primers are indicated by arrows. Solid line, filled arrow,
diamond-filled box, dark box, and open arrow are part of plasmid DNA,
part of T7 gene in pET17b, gst gene in pGEX-4T-1, leader sequence of
Mtx1, and truncated Mtx1, respectively.
B. Promdonkoy et al.: Cloning of Mosquitocidal Toxin from B. sphaericus 2297 85
mtx1 of B. sphaericus SSII-1 [17] and LP-1G [14],
leading to four amino acid changes (H74Y, F185L,
V470I, and R799Q).