Spore laccase from Bacillus amyloliquefaciens was evaluated for its dye decolorization capacity. More than 65%
of the tested dyes were decolorized by the spore laccase in the presence of acetosyringone after 6 h. The spore
laccase was entrapped in calcium alginate beads, and it could decolorize 99% of indigo carmine after fifteen
cycles of repeated use. About 90% of simulated dye effluent was decolorized by the immobilized enzyme at
pH 9.0 with 0.5 mM of acetosyringone as mediator. The results indicate a potential application of this
immobilized spore laccase in treatment of textile dye effluents.