The optimum pH was measured in a different buffer system as described previously. The enzyme displayed appropriate activity in a broad range of pH, from 3 to 11, with maximum activity at a pH of 5.0.
The enzyme achieved 84% of activity in the pH range of 4.5– 6.0, and showed 94% of activity at pH 5.0 which indicated that the optimum pH for the enzyme could be considered at pH 5.0