Production of fungal mash
Waste cakes with a particle size of 1.2 to 2.0 mm were used as
sole carbon source for producing an enzyme cocktail using solid
state fermentation in which the initial moisture content was
adjusted to 70% (wb) with 0.1 M phosphate buffer (pH 7.9). After
sterilization by autoclaving at 120 C for 20 min, the flasks were
cooled down and then inoculated with A. awamori to obtain a spore
concentration of 106/g substrate and the contents were mixed
thoroughly with a sterile spatula. Such mixture (10 g) was distributed
into Petri dishes and incubated at 30 C for 6 days under
stationary conditions. Fungal mash, i.e. the GA-rich fermentation
solids were obtained at the end of the fermentation. This fungal
mash indeed was a cocktail of various crude enzymes, and was
directly employed to hydrolyze mixed FW without further separation
of produced enzymes.
2.3. Food