Determination of hydrogen peroxide scavenging
activity—The H2O2 scavenging activity was evaluated
based on the scavenging of H2O2 in ABTS-peroxidase
system22. The reaction mixture consisted of 20 of
TSCEs at various concentrations, 20 of 10 mm
H2O2 and 100 of 0.1 M phosphate buffer (pH 5)
were incubated at 37 for 5 min. The 30 of
1.25 mM ABTS (freshly prepared) and 30 of
peroxidase (1 U/mL) were mixed successively, and
the mixture was incubated at 37 for 10 min. The
reduction of the blue-green colour was measured at
405 nm using a microplate reader. H2O2 scavenging
activity was calculated using the following equation: