4. Conclusion
A training exercise to determine niacin in cereals was developed for laboratories in the APFAN network. The data reported by the majority of analysts were very encouraging, especially as this was the first attempt at the method for many of them. Several analysts demonstrated proficiency with this assay, whilst others gained experience and would require further training to achieve proficiency. The authors did not expect to get the level of agreement between the data as expected for proficiency or collaborative trials, which are usually conducted between laboratories with a proven track record in the methodology, as most of the analysts were unfamiliar with the methodology. It would benefit all participants if a further batch of proficiency samples could be distributed perhaps after individual laboratories have obtained the necessary materials through their own suppliers. The development of an APFAN reference material would also be of benefit to participants.
The training programme also provides evidence that the method which has been developed by AGAL staff, but largely untested outside AGAL, is sound in principle and suitable for adoption in laboratories experienced in the use of HPLC. A more rigorous collaborative study involving a larger pool of laboratories is required before adoption as an approved internationally recognised method could be considered