analysis was achieved on a Hypersil C18 column (250 4.6 mm, 5 lm) from Agilent Technologies Inc.,Newcastle, DE. The mobile phase: CH3CN-H2O (85:15, v/v); the flow rate: 1 ml/min; the sample volume of injection: 20 ll; the wavelength of detection: 254 nm and the column temperature: 15 C. The extraction yield of rutin was calculated based on the integration of the chromatographic peak areas.