After evaporation the lipid residue could be conveniently
collected into this cone by washing the flask with small
amounts of solvent. The total lipid extracts were collected
in screw cap vials (9 ml capacity) and sealed with
polyethylene liners. Prior to use both liners and caps
were extracted with hot chloroform-methanol 2 :l.
Samples of lymph and tissue homogenates were treated
in an identical manner.