Present study is carried on performance of different isolates against Colletotrichum graminicola causing anthracnose of sugarcane shows different result in antagonistic study.
Antagonistic disease is controled by non-pathogenic fungi. Total 24 isolates are tested for antagonistic activity and positive result shows by 21 isolates pathogenic fungi. Antagonistic activity line is observed clear in position. Plus or square signaling is very good indication of result. The redial mycelia growth of the isolates (non-pathogenic fungi) on cultural petridisc found differed from each other.