Isolation of andrographolide: Attempts to provide plant extracts containing high level of its major active compound are being widely developed.
Andrographolide was a major and most active compound in A. paniculata leaves. In the present study, ethanolic extract of A. paniculata was fractionated gradually to provide high level of its active compound andrographolide.
n-hexane was used in the step to eliminate chlorophyll, lipid and other disturbing materials