Chlorophyll Measurement
Chlorophyll content was determined by [19] with mod- ifications: One hundred mg of fresh leaves were homo- genized with 1.7 ml of acetone 80% buffered with 2.5 mM sodium phosphate (pH 7.8), vortexes for 15 minutes and then centrifuged at 4˚C for 15 minutes at 3000 rpm. Subsequently, absorbance was measured at 663 and 645 nm.
Acetone 80% was used as a blank control.
The chlorophyll concentrations were calculated using the formula given by [20].