The procedures used for the separation of blood lymphocytes and marrow lymphoid
cells, for conjugation of the IgG fractions of the antisera with rhodamine or fluorescein,
for the detection of S.Ig by staining in the cold of living cells in suspension, and for the
labeling of intracytoplasmic lg on fixed cell smears, have been previously described.15’16’20
In order to study both surface and intracytoplasmic Ig in the same cell preparation, the
cells treated with rhodamine labeled antiserum were flattened on slides, fixed, and
stained again with fluorescein conjugates
The procedures used for the separation of blood lymphocytes and marrow lymphoidcells, for conjugation of the IgG fractions of the antisera with rhodamine or fluorescein,for the detection of S.Ig by staining in the cold of living cells in suspension, and for thelabeling of intracytoplasmic lg on fixed cell smears, have been previously described.15’16’20In order to study both surface and intracytoplasmic Ig in the same cell preparation, thecells treated with rhodamine labeled antiserum were flattened on slides, fixed, andstained again with fluorescein conjugates
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