2.5. Statistical analysis
All extraction assays were carried out in triplicate and a duplicate
of each extract was analysed. Results were expressed as
means ± standard deviation (SD). One-way analysis of variance
(ANOVA) was carried out by Statgraphics Plus software, version
2.1 (Manugistic, Inc., Rockville, MD, USA). The comparison of
means was performed by Tukey test. Statistical differences were
considered to be significant (P 6 0.05).