ABSTRACT The present work comprises the amylase enzyme production by isolated amylase producing microorganism and its industrial application. To isolate amylase producing strain, three samples were collected from different sources, soil sample, rotted potato and spoiled food waste. All isolated colonies were screened by activity zone techniques with iodine solution. Out of 12 bacterial strains, only 5 bacterial colonies showed positive results for amylase production. Amylase enzyme activity of bacterial strain-2 was found maximum (1.48IU/ml) at pH 6 and the activity decreased with increase in incubation period. Amylase enzyme used for starch degradation studies from bacterial strain 2 showed better results than commercial enzyme at same dose at pH 6.5 at 500C. Enzyme treated substrate ( ararote) showed better properties when compared to control ararote