Phylogenetic trees were also
constructed by using the maximum-likelihood (Cavalli-
Sforza & Edwards, 1967) and maximum-parsimony (Kluge
& Farris, 1969) methods with PHYLIP version 3.65 as
described previously (Endo & Okada, 2006). The 16S rRNA
gene sequences determined for the three isolates were
compared with each other and the sequence of strain F9-1T
was used to search for sequence similarity using the DDBJ
database. Approximately 1450 bp of the 16S rRNA gene
sequences of the novel isolates and related species were
used to construct the phylogenetic trees. The three new
strains shared 99.9% sequence similarity based on the 16S
rRNA gene. The highest gene sequence similarities to strain
F9-1T were recorded with L. lindneri and L. sanfranciscensis
(95.4% and 93.7 %, respectively).