In summary, the present work demonstrates the establishment of a promising plant in vitro regeneration system of C. hystrix. The appropriate concentrations of PGRs for optimum shoot and root organogenesis were determined and the occurrence of leaf senescence was successfully overcome through the inclusion of AgNO3 in the shoot regeneration and rooting media. The combination of loamy soil: Peatgro: sand (1:1:1) was chosen as the most suitable potting mixture for acclimatization.