Tourism attractions are an important element of a destination and, therefore, important in destination satisfaction formation. It is often the case that the core of destination attraction system consists of protected environments with their inherent natural (zoological, botanical, ecological, geological, geomorphologic, biodiversity) or cultural values (historical, heritage (Lundmark & Müller, 2010). Consequently, it also means that management of such environments must meet two contradictory goals: restrict human impact on these environments and, at the same time, make them accessible for visitors (Marion & Reid, 2007; Spilanis & Karayiannis, 2009). Th is is a challenge for, both, tourism and nature/heritage conservation management (for detailed review see Navrátil, Pícha, Rajchard & Navrátilová, 2011). Given the importance of tourism attractions for the overall destination satisfaction and, in particular, the prevalence of protected natural and heritage sites in the tourism attraction system of many destinations, the aim of this paper is to assess the impact of antecedents of tourism satisfaction in the context of nature-based tourism.