2.4. Microbial oil fermentation
The fermentation was carried out in a 250-mL conical flask containing 100 mL of medium at 28 oC and 180 rpm. All fermentations were performed under the conditions described in Section 2.2 for 10 days. Lactose concentrations of 4.5%, 8.0%, 12.0%, and 16.0% were used to determine the effect of initial lactose concentration on microbial oil production in DP-WP medium.