. One half of the root system was planted in
a separate pot and transferred to a climate chamber. Each half of
the plant root was artificially wounded and inoculated sequentially, 4 and 6 days after transplanting with 40 mL and 20 mL
antagonistic and R. solanacearum suspensions, respectively. Each
plant was monitored and disease symptom development was
H. Kurabachew, K. Wydra / Biological Control 67 (2013) 75–83 77recorded. Five days post inoculation, quantification of the pathogen
population in mid-stems was performed as described above.