2.1.3. pH
Optimum pH for most algae species is between 7 and 9 [7]. According to experiment conducted by Azov (1982)
highest levels of dry matter production were achieved from cultures maintained at pH 7.5. During the growth of
algae the reaction of cultivation medium is changing. Absorption of CO2 in the process of photosynthesis increase
pH even up to 11. This should be kept in mind and pH supposed to be maintained at stable level e.g. through
supplementation with CO2.
2.1.4. Nutrient Medium
The nutrient medium is a mixture of various nutrient substances used in culturing of specific organisms [8].
Unriched – providing the best effects of multiplication of algae biomass due to the rich composition of nutrients
e.g. Knop’s medium
Minimal – containing only such elements that are necessary to sustain the basic physiological function
(Baslerowa-Dvorakova medium)
Simple – containing a large amount of nutrients, but guaranteeing only the growth of less demanding organisms.