2. Material and methods
2.1. Sample collection, culture and maintenance Nannochloropsis sp. and Tetraselmis sp. were isolated from the
South China Sea and maintained at the laboratory of Faculty of Fisheries and Aqua-Industry, Universiti Malaysia Terengganu. The pure Nannochloropsis sp. and Tetraselmis sp. was cultured in Con-way culture medium (Tompkins et al., 1995). Sub-culturing was done every two weeks to maintain pure and healthy stock culture.Pure seed culture of Nannochloropsis sp. and Tetraselmis sp. were maintained at 30 ppt salinity in Conway medium.