Fruit and vegetable processing produces a considerable amount
of by-product, which is a problem since the plant material is usually
prone to microbial spoilage and is commonly used in animal
feed or fertilizer. In this study, the antioxidant activity of water
and ethanolic extracts from TJP has been evaluated in a series of
in vitro tests: free radical (DPPH, Alkyl and Hydroxyl) scavenging
activity, reducing power assay, oxygen radical absorbance capacity,reducing antioxidant potential (FRAP) assay and ABTS radical scavenging
assay. According to our study, 70% ethanolic extracts from
TJP exhibit strong antioxidant activity and protective effect against
t-BHP-induced oxidative stress.
In conclusion, ethanolic extract from TJP showed a protective
effect against t-BHP-induced oxidative damage by the increasing
cell viability and reducing cellular ROS in Chang cells. TJP ethanolic
extract also possesses promising protection against t-BHP-induced
loss of membrane potential. Further studies are ongoing for the
isolation and identification of individual phytoconstituents responsible
for the biological activity of T. japonica pericarp and also
in vivo studies are needed for better understanding of their mechanism
of action.