The fresh leaves and branches (15 kg) were extracted with
EtOH at room temperature, four times, to give an extract (980
g), which was suspended in H2O and partitioned with Et2O.
The H2O layer was concentrated in vacuo and chromatographed
over Sephadex LH-20 (H2O-MeOH, 1:0-0:1, and
then 50% acetone) to give five fractions (fractions 1-5).
Fraction 2 was subjected to MCI-gel CHP 20P (0-100%