The fish samples were collected from reef tidal pools in Búzios beach, Nizia Floresta, Brazil (06°00′40′′ S; 35°06′33′′ W). In this study, 30 pooled samples of males and females of adult damselfish were used, with mean standard length of 6.95 (±0.94) cm and mean body weight of 10.76 (±2.78) g. Each specimen was ventrally incised to expose the digestive tract which was removed from the coelomic cavity and fixed in 10% formaldehyde.