Results indicated that supplementation of organic additives CW and CH improved callus growth and somatic embryogenesis in date palm tissue cultures. It was found that CW (the liquid endosperm of Cocos nucifera fruits) when added to a medium containing auxin, it can induce cell division (Goerge et al. 2008).
Coconut water was shown to have cytokinin activity by Kuraishi and Okumura (1961) and recognised natural cytokinin substances have since been isolated [9-β-D-ribo-furanosyl zeatin, zeatin and several unidentified ones but the levels of these compounds in various samples of coconut water have not been published. An unusual cytokinin-like growth promoter, 2(3-methylbut-2-enylamino)-purin-6-one
was isolated by Letham (1982). Because coconut water contains natural cytokinins, thus adding it to culture medium often has the same effect as adding a recognised cytokinin. This means that a beneficial effect on growth or morphogenesis is often dependent on the presence of an auxin (Goerge et al. 2008). Increasing the number of globular embryos by adding casein hydrolysate may be attributed to that casein as a simple protein decomposes intoglucosamine and galactosamine or mannosamine as well as simple sugars linked with proteins by covalent bonds or by glycoside bond with hydroxyl group