The results obtained indicate that the portion of COI used as a DNA barcode effectively discriminates among black
fly species. DNA barcoding studies on insects and invertebrates have shown maximum intraspecific variation
ranging from 3 to 3.9% (Ball et al. 2005; Cywinska et al. 2006; Smith et al. 2006; Carew et al. 2007). However, smaller
maximum values have been observed in other taxa, although in most cases, these were characterized by limited sampling