2.4. pH and instrumental color evaluation
For determination of the pH, 10 g of sample was homogenized
with 50 ml distilled water and the pH value was measured using a
digital pH-meter (HM-5S; TOA Electric Industrial Co. Ltd., Tokyo,
Japan). The effect of MOL on color properties (L*, a*and b*) of
cooked sausages was evaluated using a chroma meter (5104, No:
453, WPA Linton Cambridge UK).
2.5. Microbial analysis
Frozen samples were thawed to room temperature (28 C) and
10 g of each samplewas homogenized with 90 mL buffered peptone
and was properly blended.1 mL dilutions were inoculated onto TPC
petrifilm (PetrifilmR 3M) to obtain Total Plate Count and incubated
at 30 C for 48 h. Furthermore, 1 ml dilutions were inoculated on to
E coli petrifilm (3M™ Petrifilm E coli/Coliform count plates) and
Baired Parker medium (Oxoid, UK) to obtain E. coli and S. aureus
count respectively at 37 C for 24 h.