Crude extract preparation
Mangosteen (Garcinia mangostana L.) were purchased from a local grocery store in
Bangkok, Thailand. The fresh fruit peels were chopped into small pieces and extracted with 95%
ethanol for three days, thrice, at room temperature. The filtrates were pooled and concentrated by
rotary evaporator at 40 °C. The obtained semisolid extracts were kept in a desiccator at 4°C until
further used.
2. Formulation of anti-acne gel from mangosteen crude extract
32 factorial design was employed for optimization. Two variables included content of
Carbopol Ultrez-10 and mangosteen crude extract. The levels of Carbopol Ultrez-10 were 0.3, 0.5
and 0.7% and mangosteen crude extract were 0.5, 0.75 and 1%. Nine Samples of anti-acne gel were
prepared.
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