blood glucose was estimated using on-call plus blood glucose test strips at various time periods after giving the oral glucose load. blood glucose value before giving glucose is considered as 0 minute value. Results are expressed as mg/dl.Radioimmunoassay of insulinSerum insulin was assayed using 125I-labeled radioimmunoassay kit obtained from DiaSorin. The limited of detection is the percentage cross-reactivity of insulin antibody to rat insulin was 100% and to c-peptide was <0.01% . Intra-assay coefficient of variation was <10.8%. Results are expressed as 2.10 serum lipid profile and liver and kidney marker enzymesSerum cholesterol , triglycerides , low density lipprotein (LDL) , high density lipoprotein (HDL) , alkaline phosphatase (ALP) , aspartic transaminase (AST) were assessed using assay kits purchased from spinreact, spain
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