Pathologic findings
At necropsy, the lungs of affected pigs from the two case
farms failed to collapse and were diffusely mottled, red
to purple, and rubbery (Fig. 1a). Tissue samples from
each lung lobe were fixed in 10 % neutral buffered formalin
overnight at 4 °C, dehydrated in a graded ethanol
series, embedded in paraffin wax, sectioned at 3 μm
thickness and stained with hematoxylin and eosin reagent.
Histopathologically, purulent bronchopneumonia
that is characterized by infiltration of monocytes, lymphocytes
and a few number of neutrophils and sever
congestion was seen in the lungs. The inflammatory cells
were infiltrated in alveolar spaces and walls and sometimes
in the lumen of bronchioles, which resulted in destruction
of the lung parenchyma (Fig. 1b). Written
informed consent was obtained from the farm owners
for the publication of the evaluation data and any accompanying
images.