2. Material and Method
Experimental fish used were red Larasati tilapia (O.niloticus) seeds from genetic improvement 5th generation
(F5) of selective breeding (6) with length of 3-5 cm and range weight between 0.70 – 1.3 grams approximately. A
complete random design method was used in this research. There were 5 treatments and each treatment was
replicated 3 times. The treatments were different saline media, i.e. A (0‰); B (15‰), C (20‰), D (25‰) and E
(30‰). Data collected were survival and growth rate and were analyzed using MSustat ver. 4.11 and analyzed
descriptively. Fish seeds were maintained in a plastic container with volume of 20 liter and density of 20 fish/container. Prior
to treatment, the fish were acclimatized with saline media. Salinity acclimation of 1-2‰ was done everyday starting
from salinity 30‰, 25‰, 20‰, 15‰. The data collection were started when the fish were stable. This research was
carried out on March-August 2012 at Working Unit of Fresh Water Fish Seed Centre, Janti-Klaten, Central Java