The suspension (1 ml) was serially diluted in
9 ml of the Ringers' solution further to 10À6. One ml from each
dilution was cultured on Ohio Agricultural Station agar (OAESA)
and potato dextrose agar (PDA) and incubated for 5e7 days at 30 C.
After incubation, fungal colonies were counted macroscopically
using a colony counter.