Larval survival-----During the first experiment, larval survival rates resultedin 0% on culture day 5 in treatment Artemia axenic (Art axe)(Fig. 1A). The addition of yeast (Y) on Artemia culture resultedin higher survival rate (76% at the end of culture day 6).Treatment “Artemia with PS” resulted in low survival rate (8%on culture day 5) (Fig. 1A) and showed difference with controltreatment (Art + Y) (P < 0.05). When yeast (Y) and bacteria(PS) were used, Artemia culture presented a high survival rateand no significant difference was found in comparison to thecontrol treatment (P > 0.05) (Fig. 1A). In presence or absenceof yeast, Artemia nauplii challenged with PH and VA resultedin a low larval survival (0 % on culture day 5). Compared withcontrol treatment, a significant difference was improved(P<0.05) (Fig. 1B). Treatments Artemia wih bacteria(Art+PS+PH and Art+PS+VA) and traetment with yeast andbacteria (Art+Y+PS+PH and Art+Y+PS+VA) resulted in lowsurvival rate (0% on culture day 5) and no additional protectionagainst PH and VA was noted in comparaison to the controltreatment (P<0,05) (Fig.1C).---In the second expriment, results showed an improvement ofsurvival rate in treatment Artemia with yeast (Y), with bactria(PS) and Artemia with yeast (Y) and bacteria (PS), but nosignificant difference was found (P > 0.05) (Fig. 4 A and B). Itwas demonstrated that when Artemia nauplii were fed duringthe first three days and challenged with PH and VA, the
survival rate was not affected under treatments when yeast (Y)
was used only or associated with bacteria (PS). No significant
difference in comparaison with control treatment was noted (P
> 0.05) (Fig. 4 A, B and C).
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