Trimmed and sterilized explants were inoculated in MS media
supplemented with different combinations and concentrations
of growth regulators. Cultures were observed daily. Subculturing
was done every 4 weeks to enhance response of
shoot-tips to the various treatments. From the multiple microshoots
induced in the multiplication stage, individual shoot
buds of uniform height (approx.1cm) were cut and separated
from the various multiplication media. Cutting of the multiple
micro-shoots into individual shoot buds was done under
laminar airflow with the help of sterilized forceps and blades
and were subsequently transferred into the growth and
development media. Plantlets of uniform height of 5cms with
2-3 leaves were selected for the experiment and the roots
were trimmed under aseptic condition. The culture materials
were kept in culture room maintained at 25±2ºC and 60-70
% RH under 12 hours light (intensity of 3000 lux, fluorescent