EMB Agar was used for the enumeration of E.
coli. All the tubes of E.C. broth showing gas were sub cultured on EMB agar plates and incubated at 35°C for 18-24 h. Positive plates contained typical colonies with green metallic sheen colour were inoculated on PCA slants (plate count agar) and incubated at 35°C for 18-24 h and were identified biochemical and also by kits (E. coliO157:H7 latex test reagent kit Pro Lab. Canada).